Aleuria aurantia (AAL) (FITC)

Cat# A1355-05-1mg

Size : 1mg

Brand : US Biological

Contact local distributor :


Phone : +1 850 650 7790


Conjugate
FITC

Grade
Molecular Biology Grade

Applications
IF IHC

Shipping Temp
Blue Ice

Storage Temp
-20°C

mushroom
Lectins are derived from the extracts of plants, animals, viruses and microorganisms and are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms.

Lectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose.
isolated from Aleuria aurantia mushrooms

This lectin is a dimer of two identical subunits of about 36,000 daltons each with an isoelectric point of about pH 9. Unlike Ulex europaeus and Lotus tetragonolobus lectins which prefer (a -1,2) linked fucose residues, Aleuria aurantia lectin binds preferentially to fucose linked (a -1,6) to N-acetylglucosamine or to fucose linked (a -1,3) to N-acetyllactosamine related structures.

Inhibiting/Eluting Sugar:
100-400mM L-fucose

Sugar Specificity:
Fucose, Arabinose

Applications:
Suitable for use in Immunofluorescence. Other applications not tested.

Working Concentration:
5-20ug/ml

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 6 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Source
Mushroom

Concentration
~1mg/ml

Form
Supplied as a liquid in 10mM HEPES, pH 7.5, 0.15M sodium chlroide, 0.08% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).


Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.