Specifications

Product Data
Clone Name	1C2
Applications	ELISA, WB
Recommended Dilution	ELISA. Western blot: 1/1,000-1/3,000.
Host	Mouse
Isotype	IgG2b
Clonality	Monoclonal
Immunogen	Recombinant Streptavidin (25-183 aa) purified from E. coli
Specificity	The antibody recognizes Streptavidin, a tetrameric protein secreted by Streptomyces avidinii.
Formulation	PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol State: Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Protein-G affinity chromatography
Conjugation	Unconjugated
Storage	Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Database Link	UniProt ID P22629
Background	Streptavidin binds tightly to a small growth factor biotin. It finds wide use in molecular biology through its extraordinarily strong affinity for the vitamin biotin; the dissociation constant (Kd) of the biotin-streptavidin complex is on the order of ~10-15 mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.
Synonyms	StAv
Reference Data