Reagents and instruments for immunology, cell biology and molecular biology.

HiFliQ pre-packed FPLC Columns

Available in 1 ml and 5 ml HiFliQ column sizes with high capacities

The HiFliQ columns are pre-packed and ready to use

with :
- Pre-charged Nickel-NTA and Cobalt-NTA Agarose resinfor affinity purification of poly-histidine tagged recombinant proteins by IMAC (Immobilized Metal Ion Affinity Chromatography).
- Glutathione coupled Agarose resinfor rapid affinity purification of Glutathione S-transferase (GST) -tagged proteins under native conditions.
- Protein A and Protein G Agarose FF resinfor rapid antibody purification from serum, ascites and tissue culture supernatants.
- Heparinfor for rapid affinity purification of native or recombinant proteins NEW


HiFliQ Cobalt-NTA	HiFliQ Nickel-NTA	HiFliQ GST	HiFliQ Protein A and Protein G	HiFliQ Heparin NEW

Available in 1 ml and 5 ml HiFliQ column sizes with high capacities
High binding capacity - Minimal ion leakage - High ligand density
Compatible with all common chromatography HPLC and FPLC instruments (including ÄKTA FPLC's), and low pressure pumps and syringes using an appropriate adaptor. Also compatible with a wide range of reducing agents, detergents and other buffer additives.

Specifications

Column volume: 1 or 5 ml resin

Column construction: Polypropylene

Flow rate: 1 ml/min (1 ml), 1-5 ml/min (5 ml)

Max pressure: 0.5 MPa (72 psi)

Universal 10.32 (1/16'') UNF threads: Inlet Female/Outlet Male

Dimensions: 15 x 80 mm (1 ml), 23 x 80 mm (5 ml)

Resin : Super Ni-NTA Agarose and Super Co-NTA Agarose (Base Matrix: Agarose)
Ni-NTA capacity: 50-75 mg (per 1 ml resin) Co-NTA capacity: 40-50 mg (per 1 ml resin) more info

Resin : Glutathione Agarose (Base Matrix: Agarose)
GST capacity: 10 mg (per 1 ml resin) more info

Resin : Protein A Agarose FF and Protein G Agarose FF (Base Matrix: Agarose)
Protein A capacity (hIgG): 30 mg (per 1 ml resin) Protein G capacity (hIgG): 20 mg (per 1 ml resin) more info

Resin : Porcine Heparin Agarose HP Resin
Heparin capacity: 3-5 mg (per 1 ml resin)

Designation	Cat#	Columns Size	Size (columns)
Co-NTA FPLC Columns	HiFliQ1-CoNTA-1	1 ml	1
	HiFliQ1-CoNTA-5	1 ml	5
	HiFliQ5-CoNTA-1	5 ml	1
	HiFliQ5-CoNTA-5	5 ml	5
Ni-NTA FPLC Columns	HiFliQ1-NiNTA-1	1 ml	1
	HiFliQ1-NiNTA-5	1 ml	5
	HiFliQ5-NiNTA-1	5 ml	1
	HiFliQ5-NiNTA-5	5 ml	5
GST FPLC Columns	HiFliQ1-GST-1	1 ml	1
	HiFliQ1-GST-5	1 ml	5
	HiFliQ5-GST-1	5 ml	1
	HiFliQ5-GST-5	5 ml	5
Protein A FPLC Columns	HiFliQ1-PA-1	1 ml	1
	HiFliQ1-PA-5	1 ml	5
	HiFliQ5-PA-1	5 ml	1
	HiFliQ5-PA-5	5 ml	5
Protein G FPLC Columns	HiFliQ1-PG-1	1 ml	1
	HiFliQ1-PG-5	1 ml	5
	HiFliQ5-PG-1	5 ml	1
	HiFliQ5-PG-5	5 ml	5
Heparin FPLC Columns - NEW	HiFliQ1-HEP-1	1 ml	1
	HiFliQ1-HEP-5	1 ml	5
	HiFliQ5-HEP-1	5 ml	1
	HiFliQ5-HEP-5	5 ml	5